Reporter
beta-galac
Part:BBa_K823019:Design
Designed by: Simon Kelterborn Group: iGEM12_LMU-Munich (2012-08-15)
LacZ (RBS+Freiburg standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We constructed the part in the Freiburg standard (assembly 25) for inframe fusion-proteins. There is a Shine-Dalgarno Sequence included between the XbaI and the NgoIV restriction site. It can be used for both N and C terminal fusions.
Source
Bacillus subtilis reporter vector pAC6.
References
[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke]